Composite

Part:BBa_K1660010:Design

Designed by: Dai Yuanyi   Group: iGEM15_BNU-CHINA   (2015-09-08)


RFP+RBS+attB+P+attP+RBS+GFP+Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 933
    Illegal NheI site found at 956
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 880
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 900
    Illegal BsaI.rc site found at 983
    Illegal BsaI.rc site found at 1684


Design Notes

We add attB and attP which are flanked outside the promoter.


Source

From the register.

References